Eva Permaul at Georgetown University asks: >>We just had a request for Gallocyanine staining. Does anyone do this? Can you share your protocol? Where do you buy your reagents?<<
Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance. Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it? Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
