We used the stain to stain nissl substance in mouse lumbar spine. Purchased the dye from sigma - below is from the SDS Sheet
Product name : Gallocyanine Product Number : 124508 Brand : Sigma-Aldrich CAS-No. : 1562-85-2 The procedure in Bancroft-Gamble uses Chrom Alum - which is chromium potassium sulfate as the mordant Chrom Alum - 5 gm dH20 - 100 ml Gallocyanine - 50 mg The chrome alum is dissolved in the distilled water, the gallocyanine is added and the solution slowly heated until it boils. Boil for 5 minutes. Cool to room temp adjust the volume back to 100 ml. Filter before use The stain is easy - place in staining solution for 18-48 hours, rinse in water, dehydrate, clear and mount. B&G states results are blue but If I remember correctly it turned out to be a bluish grey in color. I tried to see if I had any images but I could not find any, we did this back in 2006 so it was some time ago. If you search the histonet archives there is an older post of mine looking for some help on this stain, there is a nice response back from Dr. Kiernan explaining a bit more about the dye and staining. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell [email protected] www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bob Richmond via Histonet [mailto:[email protected]] Sent: Thursday, August 18, 2016 6:02 PM To: [email protected] Subject: Re: [Histonet] Gallocyanin Liz Chlipala at Premier Laboratory in Boulder CO writes: >>We have run this stain [gallocyanin] before, purchased the reagent >>from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble.<< What stain? What mordant (chrome, aluminum, none)? To stain what? I think the person originally posting needs to extract this information from the person who wants the stain done. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
