Develop your own data.
Check your early steps first. Tissue loss can be simple -a function of poor 
fixation, dirty processing or rush processing or slightly large tissue w 
slightly dirty reagents.

Airdry wet slides before annealing on hot plate. 
Check your baking/annealing. Some tissues require a bit more. 

We do not use that stainer.

Check your heat antigen retrieval against a manual retrieval.
Skin can handle 90 degrees for twice as long as 98. 98 degrees may cause 
excessive collagen loss. 

Steve A. McClain, MD

> On Sep 16, 2016, at 13:25, "histonet-requ...@lists.utsouthwestern.edu" 
> <histonet-requ...@lists.utsouthwestern.edu> wrote:
> Wendy

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