Develop your own data.
Check your early steps first. Tissue loss can be simple -a function of poor
fixation, dirty processing or rush processing or slightly large tissue w
slightly dirty reagents.
Airdry wet slides before annealing on hot plate.
Check your baking/annealing. Some tissues require a bit more.
We do not use that stainer.
Check your heat antigen retrieval against a manual retrieval.
Skin can handle 90 degrees for twice as long as 98. 98 degrees may cause
excessive collagen loss.
Steve A. McClain, MD
> On Sep 16, 2016, at 13:25, "histonet-requ...@lists.utsouthwestern.edu"
> <histonet-requ...@lists.utsouthwestern.edu> wrote:
Histonet mailing list