Wendy Develop your own data. Check your early steps first. Tissue loss can be simple -a function of poor fixation, dirty processing or rush processing or slightly large tissue w slightly dirty reagents.
Airdry wet slides before annealing on hot plate. Check your baking/annealing. Some tissues require a bit more. We do not use that stainer. Check your heat antigen retrieval against a manual retrieval. Skin can handle 90 degrees for twice as long as 98. 98 degrees may cause excessive collagen loss. Steve A. McClain, MD > On Sep 16, 2016, at 13:25, "histonet-requ...@lists.utsouthwestern.edu" > <histonet-requ...@lists.utsouthwestern.edu> wrote: > > Wendy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
