Lets see if I’m understanding you correctly.
1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are
the free-floating sections?
2) Are the tissue fixed? If so, with what? & For how long?
3) You want to stain the unknown whole mount using various histochemical stains
or IHC stains?
4) Can you please explain why after staining you need to reprocess for
Cause once these mystery sections are processed you'll have to cut them using a
I can maybe help you, but your going to have to provide more detail
information. Also provide your
Memory & Aging Center
> On Sep 21, 2016, at 12:38 PM, Caroline Miller via Histonet
> <email@example.com> wrote:
> Hi there Histonetters!
> I am currently working through some issues of unprocessing tissue to then
> restain 'whole mount' with various stains, and then reprocess back to
> I am running into issues of tissue being brittle and scratchy tissue after
> reprocessing and sectioning.
> Can anyone offer any advice on kinder dewaxing procedures and / or
> additives to the unprocessing / processing fluids that might help in the
> resulting piece of tissue?
> I have currently been putting it through the cleaning cycle on the
> processor, so standard xylene then alcohol treatment.
> thanks in advance for your advice!
> Caroline Miller (mills)
> Director of Histology
> 415 2187297
> Histonet mailing list
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