I am attempting to stain 30-40 micron thick undecalcified bone sections
embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS)
with a Van Geison counter stain.
My first attempt at this stain resulted in a very faint stain from the RBS (I
could see very faint blue/green staining of osteoid/soft tissues) and a very
intense dark pink stain from the counterstain. I was wondering if anyone knows
of a way to intensify the staining of the RBS? I heated the stain to 55-60
degrees Celsius as recommended by the manufacturer prior to staining (with a 10
minute stain duration).
Thank you in advance for your time and help.
Postdoctoral Research Associate
University of Colorado, Boulder
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