Hello everyone,

I am attempting to stain 30-40 micron thick undecalcified bone sections 
embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) 
with a Van Geison counter stain.

My first attempt at this stain resulted in a very faint stain from the RBS (I 
could see very faint blue/green staining of osteoid/soft tissues) and a very 
intense dark pink stain from the counterstain.  I was wondering if anyone knows 
of a way to intensify the staining of the RBS?  I heated the stain to 55-60 
degrees Celsius as recommended by the manufacturer prior to staining (with a 10 
minute stain duration).

Thank you in advance for your time and help.


Alicia Ortega

Postdoctoral Research Associate

University of Colorado, Boulder
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