Dear Histosearch,

I just became a member and I hope you can provide me with advice. I'm really 
curious about the effect of not dissolving Direct Red 80 in 1.3% saturated 
Picric Acid but in 0.9-1.1% alkalimetric Picric acid solution. The reason why 
I'm curious is that I want to perform a staining on paraffin slides for Sirius 
Red and in my current lab they have Picrosirius red dissolved in 0.9-1.1% 
alkalimetric Picric acid, but the samples were I want to compare them to are 
stained in Picrosirius red made with the standard protocol with saturated 
Picric Acid.

I measured the pH of the Picrosirius Red made with 0.9-1.1% alkalimetric Picric 
acid and this is ~pH 2.0 and all the Direct Red is dissolved, so can I assume 
that the staining will be comparable to Picrosirius Red dissolved in saturated 
Picric acid?

Thank you very much in advance.

Best Noortje

Noortje AM Bax, PhD | Laboratory for Cell and Tissue Engineering  | Biomedical 
Engineering | Eindhoven University of Technology, P.O. Box 513, Groene Loper, 
Gebouw 15, Gemini-Zuid 4.108, 5600 MB Eindhoven,  The Netherlands | T: 
+31-40-2474830 | F: +31 40-2473744 |E-mail:|<>

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