Dear Histosearch, I just became a member and I hope you can provide me with advice. I'm really curious about the effect of not dissolving Direct Red 80 in 1.3% saturated Picric Acid but in 0.9-1.1% alkalimetric Picric acid solution. The reason why I'm curious is that I want to perform a staining on paraffin slides for Sirius Red and in my current lab they have Picrosirius red dissolved in 0.9-1.1% alkalimetric Picric acid, but the samples were I want to compare them to are stained in Picrosirius red made with the standard protocol with saturated Picric Acid.
I measured the pH of the Picrosirius Red made with 0.9-1.1% alkalimetric Picric acid and this is ~pH 2.0 and all the Direct Red is dissolved, so can I assume that the staining will be comparable to Picrosirius Red dissolved in saturated Picric acid? Thank you very much in advance. Best Noortje Noortje AM Bax, PhD | Laboratory for Cell and Tissue Engineering | Biomedical Engineering | Eindhoven University of Technology, P.O. Box 513, Groene Loper, Gebouw 15, Gemini-Zuid 4.108, 5600 MB Eindhoven, The Netherlands | T: +31-40-2474830 | F: +31 40-2473744 |E-mail: n.a.m....@tue.nl|<mailto:n.a.m....@tue.nl%7C> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
