Once you finish the IHC procedure, the DAB reaction is very stable and you can
use Weigert's or any other iron hematoxylin.René
On Tuesday, November 22, 2016 5:29 PM, Esther C Peters via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
Could someone advise me on whether Weigert's iron hematoxylin can be used as
the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin
because that will stain mucocytes in the coral tissues we are working with. If
it can be used, would that be after all antibody steps have been done? Thank
you!
Esther Peters
Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-4444
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epete...@gmu.edu<mailto:epete...@gmu.edu>
<https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu>http://esp.gmu.edu
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