You do not have to "babysit" the procedure for it has well defined/timed steps.
You just need a timer and check the slides when required. As to procedural "dos
& donts" try to get a copy of the DAKO IHC manual.René
On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
Hello,
My dermatopathologist wants me to start hand staining immunos for MITF. I
would really like some feedback about everyone's experiences/thoughts with
manually hand staining immunos and especially with MITF. Pros and cons? I am
concerned about the amount of time this will take for a tech to babysit the
stain and also about the consistency of the quality in the end result. Your
opinions are much appreciated.
Thank you!
Jennifer
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