I have done that a lot and I don't see why it would not pick up hx or DAPI. Was there anything left on the slide? Maybe it was mounted in aqueous already??
mills On Fri, Mar 3, 2017 at 11:42 AM, Bernice Frederick via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello all. > Would there be any reason that slides stained with TUNEL (ISH) would not > pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers > for 7 minutes and then ammonia water for 1 minute. We do that rather than > rinse for 15 minutes and there are usually no issues. Could a reagent in > the stain be causing it? DAPI is used... > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
