Good morning All,

          My supervisor asked me to get opinions on the newer stand alone 
automated special stainer from Roche-Ventana is now offering so I am asking. We 
currently have the 2009 countertop model. Thank you in advance for taking you 
time to respond.

Cassandra Davis
Histology Technician
AP Laboratory

Sent: Tuesday, August 8, 2017 1:00 PM
Subject: [External] Histonet Digest, Vol 165, Issue 6

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Today's Topics:

   1. Examples of Movat on arteries? (Vivian Hou)
   2. PIN4 not working (Eddie Martin)
   3. H&E Protocols (Charles Riley)
   4. Exciting NEW Opportunities in California - Run your own   brand
      new lab and MORE!! (Pam Barker)
   5. PPE and countertop cleaning (Nancy Schmitt)
   6. BRCA-1 validation (Curt)
   7. HSV 1/11 (McCabe, Sara)


Message: 1
Date: Mon, 7 Aug 2017 18:08:58 -0700
From: Vivian Hou <>
Subject: [Histonet] Examples of Movat on arteries?
Content-Type: text/plain; charset="UTF-8"

Dear all,

I am learning the Movat Pentachrome stain currently (American Mastertech
kit) and trying to stain coronary arteries. I have looked through
literature examples but have been unable to locate higher resolution
examples of arteries.

I would greatly appreciate any example images from experienced histotechs
on what the final stain should look like on arteries. Any comments and tips
would also be appreciated!

Cheers and thank you,

V Hou
Bioengineering | Human Photonics Laboratory
e: | p: 206-221-3813


Message: 2
Date: Tue, 8 Aug 2017 02:40:18 -0400
From: Eddie Martin <>
To: Renee Fisher <>
Cc: ""
Subject: [Histonet] PIN4 not working
Message-ID: <>
Content-Type: text/plain;       charset=us-ascii

Hi Kim,

I was wondering if you could be more descriptive as to what is going wrong with 
your PIN4 stain?

It's difficult to give a suggestion when we don't have any clue as to what your 
lab is visualizing.

Is it the DAB?
Is it the AP Fast Red chromagen?
Is it reduced staining or no staining?

Kind regards,

Eddie Martin, HTL, HT(ASCP), QIHC(ASCP)
IHC Histotechnologist III
Joint Pathology Center (JPC)
Walter Reed National Military Medical Center
Bethesda, MD

> On Aug 4, 2017, at 5:06 PM, Renee Fisher <> wrote:
> Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio 
> Care Medical and P504S from Dako.  We double stain on the Leica Bond with the 
> Polymer refine Red and Polymer Refine detection. This stain has worked 
> beautifully in the past. Any suggestions?
> Thank you


Message: 3
Date: Tue, 8 Aug 2017 06:45:22 -0400
From: Charles Riley <>
To: Histo List <>
Subject: [Histonet] H&E Protocols
Content-Type: text/plain; charset="UTF-8"

Does anyone use a different staining protocol for their cell blocks from
their normal H&E?

Our pathologists are all the sudden complaining that the cell blocks are
consistently staining lighter than the  other specimens.

If you do stain differently, in what steps do you modify your protocol and
how do you modify it?

As always any responses are greatly appreciated


Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


Message: 4
Date: Tue, 8 Aug 2017 09:40:11 -0400
From: "Pam Barker" <>
To: "Histonetters Histonet" <>
Subject: [Histonet] Exciting NEW Opportunities in California - Run
        your own        brand new lab and MORE!!
Message-ID: <077a01d3104b$dbf207e0$93d617a0$>
Content-Type: text/plain;       charset="iso-8859-1"

Hi Histonetters,
How are you?  I hope you had a great weekend and are looking forward to a
fantastic week!!
I am presently on searches for some of my best clients in California that
are in need of histology professionals.

Can you help?

**Most of these positions are RELIA EXCLUSIVES**

My clients offer excellent compensation, benefits, in some cases relocation
assistance and all of these environments are great to work in.
These are permanent full time positions in busy growing labs.

? Ventura, CA -        Run your own brand new lab!!
? Pomona, CA ?       Run your own brand new lab!!
? San Diego, CA-      CLIA qual to Gross ? New Lab
? Irvine, CA  -          Histotechnician ? AWESOME PLACE TO WORK!
? Irvine, CA -           Lead Histology Tech
? Irvine, CA -            Histology Lab Manager
? Modesto, CA ?       Histology Supervisor - Brand New Position!!

The help I need is do you know anyone that might be interested in hearing
about any of these opportunities?  If so could you please forward my e-mail
to them?

If I place someone you refer, you will earn a referral fee!!

 If you are interested in any of these positions please call me ASAP at
866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at


Right Place, Right Time, Right Move with RELIA!

Thank You!
?Pam M. Barker
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:???? (407)353-5070
FAX:???? (407)678-2788


Message: 5
Date: Tue, 8 Aug 2017 15:00:32 +0000
From: Nancy Schmitt <>
To: "''"
Subject: [Histonet] PPE and countertop cleaning
Message-ID: <>
Content-Type: text/plain; charset="us-ascii"

Good Morning!
We are circling again on these two hot topic items!
Please share what you are using for PPE for histology processes and do you also 
wear for embedding/cutting?
Countertop cleaning?  We went to a purchases wipe and it is eating away the 
countertops:(  Would you please share what you are using and what your process 
is for countertop cleaning?
We are CAP inspected.

Thank you much for your input

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager

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Message: 6
Date: Tue, 8 Aug 2017 16:14:04 +0000
From: Curt <>
To: ""
Subject: [Histonet] BRCA-1 validation

Content-Type: text/plain; charset="us-ascii"

Just looking for some recommendations on tissue for validating BRCA-1, IHC... 
before I get to the pathologists... any recommendations. What I'm reading so 
far is ovarian carcinoma (serous) and normal breast, is that what you all have 
used and/or recommend?


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Message: 7
Date: Tue, 8 Aug 2017 16:17:49 +0000
From: "McCabe, Sara" <>
To: ""
Subject: [Histonet] HSV 1/11
Message-ID: <>
Content-Type: text/plain; charset="iso-8859-1"

Does anyone have any positive control blocks for HSV? Or for those that are 
buying their control material any suggestions?

Thank you in advance!
Sara J. McCabe, HT(ASCP)CM
Histology Supervisor
Penn Highlands DuBois
100 Hospital Avenue
DuBois, PA 15801
814-375-7514 Telephone-Office
814-375-3264 Telephone-Histology  Lab
814-375-3784 Fax<><>

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