Thanks everyone for all your suggestions! You've been very helpful! On Thu, Aug 10, 2017 at 6:16 PM Cooper, Brian <bcoo...@chla.usc.edu> wrote:
> See if you can get a sample of slides from Thermo Fisher. We use the > Superfrost Plus slides, and tissue loss just isn’t one of the problems we > ever have here. And like Tim Morken has said in his response, ensure > there’s no trapped water under the section. > > > > > https://www.fishersci.com/shop/products/shandon-colorfrost-plus-slides/6776214?searchHijack=true&searchTerm=6776214&searchType=RAPID&matchedCatNo=6776214CS > > > > Thanks, > > > > Brian > > > > *From:* Emily Horst [mailto:mashals...@gmail.com] > *Sent:* Thursday, August 10, 2017 2:54 PM > > > *To:* Cooper, Brian > *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL) > > > > They are ones that were prepackaged and sent to us from Dianon (Dianon is > not the lab I'm talking about). I have no idea what/who the slides are. I > just ordered new charged slides from Mercedes Medical and all my invoice > says is 45 degree, white. I haven't received them yet so I don't have a box > with info on it. > > > > It's a problem we've been having for awhile. > > > > On Thu, Aug 10, 2017 at 5:49 PM Cooper, Brian <bcoo...@chla.usc.edu> > wrote: > > So I worked at a reference lab for yeeeeeeeeeears and years and years, and > I can tell you that not all charged slides are created equally. What type > of slides are you using? > > > > Thanks, > > > > Brian > > > > *From:* Emily Horst [mailto:mashals...@gmail.com] > *Sent:* Thursday, August 10, 2017 2:47 PM > *To:* Cooper, Brian > *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL) > > > > No I am not. I air dry them and then package them up. > > > > On Thu, Aug 10, 2017 at 5:46 PM Cooper, Brian <bcoo...@chla.usc.edu> > wrote: > > Are you baking the slides before sending them out? How long, and what > temperature? > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcoo...@chla.usc.edu > > -----Original Message----- > From: Emily Horst via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, August 10, 2017 2:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC (EXTERNAL EMAIL) > > Hi everyone, > > We send our IHCs to an outside lab. I send them the unstained slides. I > cut them at 4 microns and put them on charged slides. We are having a lot > of issues with the receiving lab saying the tissue is falling off. Their > supervisor says the sections are too thick (remember 4 microns) and my > pathologist says they are not too thick. Any suggestions for resolving this > issue with the receiving lab? > > Thanks! > -- > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > -- > > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > -- > > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > -- Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
