Hi Emily , we do the same thing.  It could be the slides but I think it has 
more to do  with how you treat the sections after mounting.  The dry time and 
heat time.  I also think it has more to do with technique in the IHC lab.  
Brains, decal, and fatty tissues get more dry time.  All sections get 1 hr. 
heat at 60C .  if they dry overnight 15min heat seems to be fine for us.
E-van

-----Original Message-----
From: Emily Horst via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, August 10, 2017 5:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC

Hi everyone,

We send our IHCs to an outside lab. I send them the unstained slides. I cut 
them at 4 microns and put them on charged slides. We are having a lot of issues 
with the receiving lab saying the tissue is falling off. Their supervisor says 
the sections are too thick (remember 4 microns) and my pathologist says they 
are not too thick. Any suggestions for resolving this issue with the receiving 
lab?

Thanks!
--
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio 
Kettering, Ohio 937.297.0028 _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to