Tendons - use a tungsten carbide blade/ 50 degree.
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From: [email protected]
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Sent: Tuesday, December 5, 2017 12:00 PM
To: [email protected]
Subject: Histonet Digest, Vol 169, Issue 3
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Contents of Histonet digest..."
Today's Topics:
1. ANP.10255 (Adesuyi, Banjo)
2. Science Haiku (Amos Brooks)
3. looking for diff cell counter (Lauren Sweeney)
4. Noise Levels in Lab (Melissa Burns)
5. Microtomy of Equine Tendon Blocks (Alan)
6. Re: Noise Levels in Lab (Morken, Timothy)
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Message: 1
Date: Mon, 4 Dec 2017 19:14:53 +0000
From: "Adesuyi, Banjo" <[email protected]>
To: "'[email protected]'"
<[email protected]>
Subject: [Histonet] ANP.10255
Message-ID: <[email protected]>
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Good afternoon,
Please I am wondering whether you can direct me to
where I can get a policy for the pathologist's participation in a peer
educational program and internal audits
Best regards,
Adesupo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS, Supervisor, Histology Section
[email protected]<mailto:[email protected]>
O: 405-307-1145
M: 405-973-6363
F: 405-307-1143
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Message: 2
Date: Mon, 4 Dec 2017 20:08:59 -0500
From: Amos Brooks <[email protected]>
To: "[email protected]"
<[email protected]>
Subject: [Histonet] Science Haiku
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Anyone that heard Science Friday recently may have heard of this. I thought it
would be fun to see what others come up with. So many people think I'm an
archaeologist when I tell them I'm a Histotech. This makes for a fun elevator
pitch for our profession.
Scientists are challenged to summarize their work in a haiku. Here's mine...
Sick organs need tests
It's microscopic study
Thats histology
#flamechallenge
<https://www.facebook.com/hashtag/flamechallenge?source=feed_text&story_id=1735098133176169>
http://www.stonybrook.edu/happenings/community-outreach/35567/
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Message: 3
Date: Tue, 5 Dec 2017 13:32:39 +0000
From: Lauren Sweeney <[email protected]>
To: "[email protected]"
<[email protected]>
Subject: [Histonet] looking for diff cell counter
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Good Morning Histonet colleagues,
My PI wants me to purchase a differential cell counter and I was wondering if
anyone had any recommendations for a basic manual counter. One that offers the
ability to count 5 different cell types and dings at every 100 cells. I found
one through Fisher but it seems pricey. Does anyone know other places to get
them?
Thanks!
Lauren
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Message: 4
Date: Tue, 5 Dec 2017 13:45:40 +0000
From: Melissa Burns <[email protected]>
To: "[email protected]"
<[email protected]>
Subject: [Histonet] Noise Levels in Lab
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="us-ascii"
Does anyone monitor the noise levels in their lab? If so, what is your
protocol? Do you have a device to do this?
Thanks in advance :)
Melissa
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Message: 5
Date: Tue, 5 Dec 2017 16:03:07 +0000
From: Alan <[email protected]>
To: "[email protected]"
<[email protected]>
Subject: [Histonet] Microtomy of Equine Tendon Blocks
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="utf-8"
Greetings to all on Histonet. I have a particularly challenging question
regarding the sectioning of Equine tendon material. My colleague and I recently
received a number of TS tendon slices. Unfortunately they had been placed in
90% IDA for fixation approximately 10 years ago, where they remained up to the
point they were sent to our lab for cutting and staining.
The blocks are extremely hard, almost like undecalcified bone. They have
processed well into paraffin, but we have wrecked several disposable blades
just in facing the blocks. They have a very gritty feel on trimming even at 2
microns.
We have tried some commercial softening agents, including Mollifex, with long
immersion over days, rather than the usual hour or so, we have tried neat
fabric softener and 10% phenol in 70% IDA. We have also left some blocks in
molten paraffin for several days, as this sometimes works with very fibrous
tissues. As does prolonged chilling with ice and water.
We have managed to recover a small number of reasonable sections, at present we
are looking at 1 block = 1 blade to obtain a very few working sections.
Do any histotechs out there have any secret recipes that they are prepared to
share and have made up themselves, or a popular in-house reagent that they know
is reliable, that they have enjoyed success with in their labs, or references
to softening solutions for very difficult tissues, that may be historical or
more recent. We have, of course, asked the referring lab to place all future
freshly dissected tissues immediately into 10%NBF. Thank you for any assistance
you are able to offer.
Yours sincerely
Alan Taylor BSc(Hons). FRMS
Microtechnical Services
Exeter. UK
www.microtechnicalservices.co.uk
Sent from Mail for Windows 10
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Message: 6
Date: Tue, 5 Dec 2017 16:01:59 +0000
From: "Morken, Timothy" <[email protected]>
To: Melissa Burns <[email protected]>
Cc: Histonet <[email protected]>
Subject: Re: [Histonet] Noise Levels in Lab
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We don't but there are decibel monitor phone apps. Not sure how accurate they
are...
I've used one called Sound Meter. It shows 70+ db next to our -80 freezer and
90+ db next to the 6-foot fume hood.
In our lab with cryostats it shows 60+ and in a quiet lab, no equipment
running, it shows 50+
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
-----Original Message-----
From: Melissa Burns via Histonet [mailto:[email protected]]
Sent: Tuesday, December 05, 2017 5:46 AM
To: [email protected]
Subject: [Histonet] Noise Levels in Lab
Does anyone monitor the noise levels in their lab? If so, what is your
protocol? Do you have a device to do this?
Thanks in advance :)
Melissa
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