Be sure you are processing them on an overnight process. Even if the samples are small it takes that time to process the agar properly.
I use Histogel and learned this lesson the hard way. My samples always "shriveled" up too until someone enlightened me about the processing time. I would think agar might be the same. Just a thought, Colleen Forster HT(ASCP)QIHC U of MN On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I would try histogel instead of agar. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com<mailto:l...@premierlab.com> > www.premierlab.com<http://www.premierlab.com/> > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > From: Heather Marlatt via Histonet [mailto:histonet@lists. > utsouthwestern.edu] > Sent: Tuesday, December 19, 2017 9:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mouse bladder > > Does anyone have a good protocol for mouse bladder embedded in agar that > they are willing to share? We have mouse bladders in 2% agar and have tried > a few different protocol variations from our usual mouse but the agar keeps > shriveling up. > > Thanks > Heather > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet