Good Morning HistoNet,
Would anyone be willing to share what they use for control tissue for MMR
testing? Finding negative expressing tumor is becoming more difficult for us
to find and we're wondering if there is an alternate negative tissue that would
NordiQC recommends using Tonsil as a positive tissue control and Colon
Adenocarcinoma that has a loss of expression as a negative tissue control for
all four antibodies (MLH1, MSH2, MSH6, PMS2). Stromal cells in the negative
tissue control should have positive nuclear staining and serve as a positive
internal control. NordiQC doesn't have any recommendations for alternative
tissue to use as a negative control.
Anyone doing anything differently?
Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201
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