Good Morning HistoNet,

Would anyone be willing to share what they use for control tissue for MMR 
testing?  Finding negative expressing tumor is becoming more difficult for us 
to find and we're wondering if there is an alternate negative tissue that would 
be acceptable.

NordiQC recommends using Tonsil as a positive tissue control and Colon 
Adenocarcinoma that has a loss of expression as a negative tissue control for 
all four antibodies (MLH1, MSH2, MSH6, PMS2).  Stromal cells in the negative 
tissue control should have positive nuclear staining and serve as a positive 
internal control.  NordiQC doesn't have any recommendations for alternative 
tissue to use as a negative control.

Anyone doing anything differently?



Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201


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