Subject: [Histonet] P16 Antibody Jason, I also tried many different vendors & clones many years ago (human FFPE tissues), and the winner is from Roche/Ventana (CINtec, Cat. 9517 = 11.5ml). You can get 5ml dispenser as well.
*Madeleine Huey**, HTL & QIHC (ASCP)* Supervisor - Pathology (Histology & IPOX) El Camino Hospital, and Los Gatos Hospital 2500 Grant Rd (Rm #GC-413), Mountain View, CA 94040-4378 Phone: 650-940-7038 Fax: 650-966-9292 Email: *[email protected] <[email protected]>* On Fri, Feb 16, 2018 at 10:00 AM, <[email protected] > wrote: > Send Histonet mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Deplastifying MMA (Kusters, Ronald) > 2. Re: [EXTERNAL] Deplastifying MMA (Dorothy Hu) > 3. P16 Antibody (Jason McGough) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 Feb 2018 09:50:15 +0000 > From: "Kusters, Ronald" <[email protected]> > To: Reuel Cornelia <[email protected]>, > "[email protected]" > <[email protected]> > Subject: Re: [Histonet] Deplastifying MMA > Message-ID: <[email protected]> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Reuel, > Maybe the Kulkzer Technovit manufacturers website provides you more > information or you could contact their specialists directly through this > link: > http://kulzer-technik.de/en_kt/kt/maerkte/histologie/ > startseite_histologie.aspx > > Yours, > Ronald > > Mit freundlichen Gr??en/Kind Regards > > > Ronald Kusters > International Sales Manager Feather Brand Histotechnology > > pfm medical ag > Wankelstra?e 60 > 50996 K?ln, Germany > T +49 (2236) 9641 660 > F +49 (2236) 9641 99 660 > M +49 (171) 9144343 > > > > -----Original Message----- > From: Reuel Cornelia [mailto:[email protected]] > Sent: maandag 12 februari 2018 22:07 > To: [email protected] > Subject: [Histonet] Deplastifying MMA > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene for > several days with heat incubation at 60 degrees. This is the first time > that happened to my section for several years of doing the same thing over. > I was thinking that it was my Xylene lot number so I tried to change it but > still does not remove the plastic, and then Itried different solvent > Acetone, and Ethylene glycol Monoethyl ether but still it does not work. > Can I ask for help if anyone knows what was going on and what would be the > best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly phthalate- > 5% and perkadox 16- 0.5%. This have been my solution for years and I do not > have any problem with the removal of plastic section until now. I was > thinking that my MMA (M55909) different lot number from Sigma Aldrich may > have cause this because even I tried to use the MMA to dissolve my plastic > it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > ---------------------------------------------------------------------- > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the treatment of orthopedic conditions, certain > related neurological disorders and learning disorders, such as dyslexia. > This email transmission and/or its attachments may contain confidential > health information, intended only for the use of the individual or entity > named above. > > > > > ------------------------------ > > Message: 2 > Date: Fri, 16 Feb 2018 08:34:42 -0500 > From: Dorothy Hu <[email protected]> > To: [email protected] > Subject: Re: [Histonet] [EXTERNAL] Deplastifying MMA > Message-ID: > <CAJ2-qz7tX+hYHAcApBc3=JtcWdxOoAcvor-dPeDjD8NPW=8z4Q@ > mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hi Lori, > > Would you please tell me which company and catalog # of tetrahydrofuran > you used before? > For how many minutes you did for deplastifying in tetrahydrofuran? > Thanks in advance. > > Dorothy > > Message: 3 > Date: Wed, 14 Feb 2018 20:05:16 +0000 > From: "Garcia, Lori, M.Sc." <[email protected]> > To: "[email protected]" <[email protected]> > Cc: "[email protected]" > <[email protected]> > Subject: > Message-ID: > <MWHPR05MB29764D1B79279A8FAA9A2F3EFDF50@MWHPR05MB2976. > namprd05.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hi Reuel, > > One thing you could try is THF (tetrahydrofuran). We have used it in the > past to rapidly dissolve the MMA on slides. > > Good luck, > Lori > > > ------------------------------ > > Message: 3 > Date: Fri, 16 Feb 2018 09:31:11 -0700 > From: Jason McGough <[email protected]> > To: '[email protected]' > <[email protected]> > Subject: [Histonet] P16 Antibody > Message-ID: <[email protected]> > Content-Type: text/plain; charset=utf-8 > > We are looking to bring in P16 to do in house but are having a hard time > getting the stain to be clean. We are having issues with positive staining > on tissue that should be negative. We are working with a concentrated > antibody that we have diluted out to 1:6000 (which is 6 times the amount > suggested from vendors 1:200-1:1000). We might need to change clones or > vendors and were wondering if you could tell us what kind of p16 (clone) > you use for staining and possible what vendor you get it from? Any > information or suggestions you have would greatly be appreciated. Thank you. > > > Jason McGough, HT(ASCP) > > Operations Manager > Clinical Laboratory of the Black Hills > 605-343-2267 > [email protected] > www.clinlab.com > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 171, Issue 15 > ***************************************** > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
