Good Day, Tim:
I'll second what Terri said. My first concern with embedding a contact lens
would be that the embedding media and/or transitional solvents would attack the
lens and/and dislodge the bacteria. Unless you have a few lenses to do a method
development pilot study with, I'd shy away from a full embedding. If you had to
go the TEM route, maybe a plastic like LR White, as the dehydration doesn't
have to be as aggressive.
The SEM is probably the best tool for this sort of investigation; we've had
luck with identifying bacterial strains on morphology alone with our SEM, or at
least it got us into the ballpark enough for the investigation.
Children's Colorado has an SEM in their facility.
Cheers,
-Chris
Christopher Hayden
Electron Microscopy / Digital Imaging
LS - Specialty Laboratories
T +1 862 778 7993
christopher.hay...@novartis.com
Novartis Institutes for Biomedical Research
Date: Fri, 9 Mar 2018 19:30:27 +0000
From: "Terri Braud" <tbr...@holyredeemer.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Identification of bacteria on an acrylic lens
prosthesis
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F8B2325A46@HRHEX02-HOS.holyredeemer.local>
Content-Type: text/plain; charset="us-ascii"
I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The
scanning electron microscope (SEM) can also be useful to reveal morphological
features of isolated organisms as well as for diagnosis. There are numerous
articles citing this technique. I believe the UC Berkley has an SEM in their
lab. I hope this helps. Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:
1. Prosthetic lens processing, cutting? (Morken, Timothy)
Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?
Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for
EM, or any other way ? We have a case where they wanted EM on a lens to
identify a specific bacteria. We have never done it and it would take some
work to figure it out. Also, I told them that unless there is some morphologic
marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
Christopher Hayden
T +1 862 778 7993
christopher.hay...@novartis.com
-----Original Message-----
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu]
Sent: Saturday, March 10, 2018 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 172, Issue 7
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Today's Topics:
1. Re: Histonet Digest, Vol 172, Issue 6 "Slide labels or slide
Printer" (Brent Adams)
2. Re: Identification of bacteria on an acrylic lens prosthesis
(Terri Braud)
3. SAVE THE DATE! HISTOPALOOZA 2018 APRIL 20-APRIL 22
(Zimmerman, Billie)
4. What is the smallest diameter (easily available and cheap)
syringe? (Jorge A. Santiago-Blay)
----------------------------------------------------------------------
Message: 1
Date: Fri, 9 Mar 2018 18:48:14 +0000
From: Brent Adams <bad...@acadianagastro.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 172, Issue 6 "Slide
labels or slide Printer"
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Content-Type: text/plain; charset="Windows-1252"
I also have used Sakura Smart Write (Primera printers repackaged under the
Sakura name) slide and cassette printers since they came out. I had some
issues with the slide printers in the beginning but they have worked out all
the bugs and greatly improved the machine. I do very little maintenance other
than clean rollers on the slide printer and never had any issues with the
cassette printer. I recommend them for Small Labs for sure. They have multi
cassette holders for larger labs.
Hope that helps
Brent Adams ? BS, LPN, HT
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acadianagastro.com&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=Dzzc938ChQJl3YEQb3wQdhPZLoBnOMy2id1HRrn_bLc&e=
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Today's Topics:
1. Prosthetic lens processing, cutting? (Morken, Timothy)
2. Re: Slide labels or slide labeler (Blazek, Linda)
----------------------------------------------------------------------
Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?
Message-ID:
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Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for
EM, or any other way ? We have a case where they wanted EM on a lens to
identify a specific bacteria. We have never done it and it would take some
work to figure it out. Also, I told them that unless there is some morphologic
marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
------------------------------
Message: 2
Date: Thu, 8 Mar 2018 15:32:57 -0500
From: "Blazek, Linda" <lbla...@digestivespecialists.com>
To: Victor <victor_tob...@comcast.net>, "Vickroy, James"
<jvick...@springfieldclinic.com>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Slide labels or slide labeler
Message-ID:
<5a2bd13465e061429d6455c8d6b40e392a826e8...@ibmb7exchange.digestivespecialists.com>
Content-Type: text/plain; charset="utf-8"
I've used the Primera slide printer since it came out. I like it a lot. It
needs it's maintenance kept up like anything else but other than cleaning the
rollers there is very little else that needs cleaning.
Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com
-----Original Message-----
From: Victor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, March 07, 2018 5:59 PM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide labels or slide labeler
James,
I totally agree with your assessment of the Slidemate printers. Have not had
any experience with the newer model.
Applying slide labels shouldn?t be an issue when dealing with one block and
slide(s) at a time. Some of this could depend upon your software available to
you. Our system generated just the labels needed for the block you were
cutting, on demand. You should be able to get a nice Zebra printer for under
$700.
Another of our labs used the new Sakura/Premera printer and really like it.
Victor
Sent from Mail for Windows 10
From: Vickroy, James via Histonet
Sent: Wednesday, March 7, 2018 2:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide labels or slide labeler
We have used the older version Slidemate slide labelers from Thermofisher for
the last three years. I think putting the patient information directly on the
slide is preferred however the wear and tear on slide printers means higher
maintenance costs and more down time. Thermofisher has a newer version of the
Slidemate now that has been designed so that there is less wear and tear on the
machines. Currently when one of the old labelers breaks down we have to send
the instrument out and the cost is generally 1000 - 1500 for repair each time.
It also seems that the older machines have to be sent in at least every other
year. Our older machines have been sent in almost yearly.
We are considering switching to a slide label making system by General Data.
One of the downsides is that we have to apply the label to each slide so
therefore we introduce another step where the slides could be mislabeled. The
upside is that equipment costs are much less. A new thermal label printer
runs around 700.00 where as the instrument that imprints the slide label on the
slide runs between 12000 - 15000 per unit. We need three units.
Thermo also offers a lease system. What I need to know is what has been the
experience with the new Slidemate AS printers? What kind of maintenance
issues have you encountered? Finally please let me know your latest experiences
with both slide label printers versus slide labeling printers?
Thanks for your input.
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>
This electronic message contains information from Springfield Clinic, LLP that
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Message: 2
Date: Fri, 9 Mar 2018 19:30:27 +0000
From: "Terri Braud" <tbr...@holyredeemer.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Identification of bacteria on an acrylic lens
prosthesis
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F8B2325A46@HRHEX02-HOS.holyredeemer.local>
Content-Type: text/plain; charset="us-ascii"
I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The
scanning electron microscope (SEM) can also be useful to reveal morphological
features of isolated organisms as well as for diagnosis. There are numerous
articles citing this technique. I believe the UC Berkley has an SEM in their
lab. I hope this helps. Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:
1. Prosthetic lens processing, cutting? (Morken, Timothy)
Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?
Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for
EM, or any other way ? We have a case where they wanted EM on a lens to
identify a specific bacteria. We have never done it and it would take some
work to figure it out. Also, I told them that unless there is some morphologic
marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
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Message: 3
Date: Fri, 9 Mar 2018 19:43:06 +0000
From: "Zimmerman, Billie" <bzimm...@augusta.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] SAVE THE DATE! HISTOPALOOZA 2018 APRIL
20-APRIL 22
Message-ID:
<bn6pr03mb25946d3a4dc67341a904f832cb...@bn6pr03mb2594.namprd03.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
TGIF everyone
The Georgia Society for Histotechnology meeting will be at the Callaway Gardens
Lodge, Pine Mountain, GA More details to follow, but for now save the date!
Billie Zimmerman
------------------------------
Message: 4
Date: Sat, 10 Mar 2018 10:43:45 -0500
From: "Jorge A. Santiago-Blay" <blayjo...@gmail.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] What is the smallest diameter (easily available
and cheap) syringe?
Message-ID:
<cagbdduarmduafbytdle-35erflyzsycud7j1wvxugzpoct6...@mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"
Hi:
I am preparing a device for blowing air into a tiny, subcylindrical structure
(diameter << 1 mm) in some (non-human) organisms. Heat extruded Pasteur
pipettes are good but too fragile for my taste.
What is the smallest diameter (easily available and cheap) syringe?
If you have any constructive suggestions, please email me directly.
Cheers,
Jorge
Jorge A. Santiago-Blay, PhD
blaypublishers.com
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