Cap states, "should test a minimum of 10 positive and 10 negative tissues". Does not state "cases" but "tissues", so we used a block for positive and negative from the same case.
Thanks, Tim -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Friday, March 16, 2018 12:00 PM To: [email protected] Subject: Histonet Digest, Vol 172, Issue 13 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Leica ST5050 immunostainer (Matthew Fleming) 2. Antibody Validation CLIA (Paula) ---------------------------------------------------------------------- Message: 1 Date: Fri, 16 Mar 2018 08:00:00 -0500 From: Matthew Fleming <[email protected]> To: [email protected] Subject: [Histonet] Leica ST5050 immunostainer Message-ID: <CAHL=4t6dhjhej7mawogv0rddx27x17fxgwxghbsrowtvbw_...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Hi, I have a small dermatopathology lab and would like to introduce limited immunohistochemistry. Initially I would probably just do MITF stains with red chromogen on a few cases per week. We could do this by hand but I've noticed a number of ST5050 immunostainers for sale at very low prices. I know they're quite old but we have a number of machines old or older that continue to serve us well. But so far I haven't been able to find a manual and am concerned about finding consumables. Leica apparently stopped supporting this model some time ago. Is anyone out there still using it? Anyone have a manual? Anyone like to comment as to whether it might be usable for a limited IH operation? Thank you, Matthew Fleming, MD Fleming Dermatopathology Milwaukee, WI ------------------------------ Message: 2 Date: Fri, 16 Mar 2018 06:54:30 -0700 From: "Paula" <[email protected]> To: <[email protected]> Subject: [Histonet] Antibody Validation CLIA Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 172, Issue 13 ***************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
