I would like to hear from anyone with experience in working with the pineapple sponge and other similar types. A future project will include processing, sectioning and staining of the samples in order to identify them by viewing the arrangement of the spicules. I am wondering if cryo, paraffin or plastic would be the best method? How much fixation is necessary? If anyone has working protocols they would share, I would be so thankful.
Nancy Thomas Senior Lab Manager, Histology Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
