Hey Alan,
I use a Airfiltronix Ductless Bench Top Hood and it meets all my needs. I gross about 7500-8000 GI biopsies per year and do any hand cover slipping under this hood (which is rare if I can help it). The unit is made of clear acrylic, is see thru and is framed out in light weight aluminum. It has a clear Velcroed thick mil roll up sash which is very easy to work with. The system has two blower units on top. Both units have 2 inch carbon filters that I purchase from Mercedes Medical and replace twice a year. I did add a grated plastic secondary containment box which I bought through Grainger to place the unit on so that I could keep my formalin cube under the hood and not worry about spillage. This also elevated the work surface off the counter by 8-10 inches so you are not leaning over the work and therefore saving your back. I had this unit tested by an outside agency along with the whole lab before we started operation and then again after we started operation. Our PEL numbers for Formalin, Alcohol and Xylene were barley negligible. I have used the badge system every year since the original testing with the same results. This to me is the key to selecting any hood, does it keep the PEL numbers within exposure limits. The purchase price is around $2800.00 + filters @ $180 ea. Hope this helps and good luck. Brent Adams – BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: [email protected] <[email protected]> Sent: Thursday, May 10, 2018 12:00 PM To: [email protected] Subject: Histonet Digest, Vol 174, Issue 7 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Paraffin infiltration issue, will this impact IHC? (Greg Dobbin) 2. Histotech Position - Chandler AZ (Office Manager) 3. IHC and Histogel (Paula) 4. Re: IHC and Histogel (dusko trajkovic) 5. Re: IHC and Histogel (John Shelley) 6. Shandon Veristain Gemini Autostainer (Charles Riley) 7. benchtop fume hoods (Histology) 8. Re: Shandon Veristain Gemini Autostainer (Mike Toole) 9. Re: benchtop fume hoods (Allan Wang) ---------------------------------------------------------------------- Message: 1 Date: Wed, 9 May 2018 15:26:57 -0300 From: Greg Dobbin <[email protected]> To: [email protected], [email protected] Subject: Re: [Histonet] Paraffin infiltration issue, will this impact IHC? Message-ID: <CAHG6iQyJyH7HH2x8gibxkaA8BBEjn_=GSYhKvQ2r5nEL=qo...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Hi Merissa, As far as I am aware, insufficient paraffin infiltration would only affect sectioning. The epitopes that we we are attempting to stain with IHC are affected by pre-analytic factors such as fixation, cold ischemic time and perhaps heat (too much) but plus or minus wax should not be an issue if you are able to obtain good sections. Have you stained these poorly infiltrated specimens with H&E? Are you certain you have good sections with all tissue components represented? That would be my main concern. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ------------------------------ Message: 2 Date: Wed, 9 May 2018 14:26:22 -0700 From: Office Manager <[email protected]> To: [email protected] Subject: [Histonet] Histotech Position - Chandler AZ Message-ID: <CAKRVzfqK4raB6+J=HnKDHFYQ2H=YOheHg-eVvnk7Du0KA=b...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" *Essential functions and responsibilities:* - Ensures compliance with all local, federal, CLIA, regulations. - Performs routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. - Responsible for embedding of surgical pathology specimens. - Responsible for the sectioning and microtomy of surgical pathology specimens. - Responsible for the routine staining, histochemical staining, and immunohistochemical staining of surgical pathology specimens for microscope evaluation and analysis. - Performs routine maintenance and cleaning of equipment and troubleshoots minor equipment failures. Documents remedial actions such as repairs or repeated tests. - Adheres to laboratory?s quality control policies and documents all quality control activities. - Maintains diagnostic viability of all specimens and ensures correct patient labeling. - Unpacks orders received; dates and stocks orders, in accordance with established policies and procedures. - Maintain a clean and safe prep work area in accordance with all laboratory and safety SOP?s. - Prepares and labels necessary stains and reagents in accordance with departmental procedures, policies and standards. - Reviews and performs QC on slides before taking the slides to the pathologist. - Maintenance of accurate work records and logs (i.e., Discrepancy Logs, Maintenance Logs, Quality Control Logs etc.) - Demonstrates a commitment to service, organizational values, and professionalism through appropriate conduct and demeanor at all times. - Works collaboratively and supports efforts of team members. - Protects patient information by adhering to professional standards including the Health Information Portability and Accountability Act (HIPAA). *Knowledge and Skill:* - Must have 2+ years laboratory experience. - HT Certified preferred. - Must be precise when performing technical tasks. - Effective interpersonal skills, both in person and on the telephone. - High accuracy in work and attention to detail. *Physical demands:* - Must have manual dexterity and motor coordination. - May involve sitting, standing, walking for long periods of time and/ or sitting at the microtome for long periods of time. - Exposure to chemicals and fumes. *Benefit Package:* - Health, Dental and Vision Insurance, 401K, Paid time off, Sick time and Holiday Pay. *Required education:* - Associate?s Degree or higher *Required experience and certification:* - Laboratory: 2 years - HT Certification ASCP preferred. East Valley Gastroenterology & Hepatology Associates is an Equal Opportunity Employer. This company does not and will not discriminate in employment and personnel practices on the basis of race, sex, age, handicap, religion, national origin or any other basis prohibited by applicable law. Hiring, transferring and promotion practices are performed without regard to the above listed items. Job Type: Full-time Reports to: Laboratory Manager Schedule: 40 Hours per Week Salary: DOE FLSA Status: Hourly; Non-Exempt To apply please reply to [email protected] or fax resume to (480) 786-6996, Attn:Isaac Malagon ------------------------------ Message: 3 Date: Thu, 10 May 2018 07:13:10 -0700 From: "Paula" <[email protected]> To: <[email protected]> Subject: [Histonet] IHC and Histogel Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ------------------------------ Message: 4 Date: Thu, 10 May 2018 14:19:55 +0000 (UTC) From: dusko trajkovic <[email protected]> To: Paula <[email protected]>, "[email protected]" <[email protected]> Subject: Re: [Histonet] IHC and Histogel Message-ID: <[email protected]> Content-Type: text/plain; charset=UTF-8 We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet <[email protected]> wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 10 May 2018 14:22:07 +0000 From: John Shelley <[email protected]> To: dusko trajkovic <[email protected]>, Paula <[email protected]> Cc: "[email protected]" <[email protected]> Subject: Re: [Histonet] IHC and Histogel Message-ID: <[email protected]> Content-Type: text/plain; charset="iso-8859-1" I agree with Dusko, I have used histogel the same way and have also used it for FISH and ISH without any issues. Kind Regards! ? John J Shelley Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 ****email:? [email protected] ??****Please note email change -----Original Message----- From: dusko trajkovic via Histonet [mailto:[email protected]] Sent: Thursday, May 10, 2018 10:20 AM To: Paula; [email protected] Subject: Re: [Histonet] IHC and Histogel We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet <[email protected]> wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA _______________________________________________ Histonet mailing list [email protected] https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177&sdata=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D&reserved=0 _______________________________________________ Histonet mailing list [email protected] https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177&sdata=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D&reserved=0 ------------------------------ Message: 6 Date: Thu, 10 May 2018 10:50:52 -0400 From: Charles Riley <[email protected]> To: Histo List <[email protected]> Subject: [Histonet] Shandon Veristain Gemini Autostainer Message-ID: <caaqhb10gnz2ners016yggmr8x41bvmmh7e0j87icjkmyoc3...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 7 Date: Thu, 10 May 2018 15:25:24 +0000 From: Histology <[email protected]> To: "[email protected]" <[email protected]> Subject: [Histonet] benchtop fume hoods Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi all, Can anyone share what type of benchtop fume hoods they are using in the lab? This would be for manual coverslipping and grossing. Thanks in advance! ------------------------------ Message: 8 Date: Thu, 10 May 2018 10:43:41 -0500 From: Mike Toole <[email protected]> To: "[email protected]" <[email protected]> Subject: Re: [Histonet] Shandon Veristain Gemini Autostainer Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358CFE1F483@mail> Content-Type: text/plain; charset="us-ascii" Charles, The issue is most likely the handle for the rack, The rack has a moveable handle. Make sure the handle is securely in the upright position. It has pins and holes that should line up and click into place. If it is even slightly at an angle or not securely snapped into an upright position it may bump into the side of the stain pots (and really cause a mess). Mike -----Original Message----- From: Charles Riley via Histonet [mailto:[email protected]] Sent: Thursday, May 10, 2018 9:51 AM To: Histo List Subject: [EXTERNAL] [Histonet] Shandon Veristain Gemini Autostainer Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 10 May 2018 12:38:28 -0400 From: Allan Wang <[email protected]> Cc: "[email protected]" <[email protected]> Subject: Re: [Histonet] benchtop fume hoods Message-ID: <ca+3cvl5ecd_r_nfqxdr5ouyzxgppyq2c+gxoyz5alkjrbm2...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < [email protected]> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 174, Issue 7 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
