Jamie Watson, you need to clarify this question: >> Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor?<<
100% ethanol is perfectly OK, but it requires a lot of regulator compliance because you can drink it (though you shouldn't). The usual denatured alcohol for histology is customarily called "reagent alcohol". It contains 90% ethanol, 5% methanol, and 5% isopropanol. The BATF calls it "S3D modified". It's also possible to denature with methyl isobutyl ketone (MIBK) but it smells horrible. Alcohol denatured with acetone is available, but it'll extract your eosin - you need to know about it because an occasional manager will insist on it. All of these are equivalent from a practical viewpoint. Isopropyl alcohol (isopropanol) is of course quite different, and I have no idea how many labs use it. Bob Richmond Samurai Pathologist Maryville TN On Sat, May 12, 2018 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ethanol in tissue processing (Jamie Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 12 May 2018 16:06:01 +0000 > From: Jamie Watson <ja...@watson-home.com> > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Ethanol in tissue processing > Message-ID: > <DM3PR1001MB10690B9001286B4DCD1B3A1DED9E0@DM3PR1001MB1069. > namprd10.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > Does anyone know what percentage of labs use ethanol vs. denatured alcohol > or isopropyl alcohol on the tissue processor? > > Jamie > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 174, Issue 9 > **************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
