We use a RTU (clone EP310) from Cell Marque on the Leica Bond. Antigen retrieval is "high pH" HIER for 20 minutes.
Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Alminawi, Samira via Histonet [mailto:[email protected]] Sent: Friday, May 25, 2018 10:29 AM To: [email protected] Subject: [Histonet] LEF1 - antibody This email is from outside HHC. BE CAREFUL when opening attachments or links from unknown senders. Hi, Is anybody using LEF1 antibody and what is the recommended protocol? Thank you for help Samira Alminawi, MLT Senior- Immunohistochemistry Sunnybrook Health Sciences Centre 2075 Bayview Avenue, Toronto, ON M4N 3M5 Tel: 416-480-6100 ext:1666 e-mail: [email protected]<mailto:[email protected]> This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
