Hi Pablo
What is the name, source and catalogue number of your antibody? ( maybe you do not need to fix in Bouin's fixing fluid....do you know, as a fact, that Bouin's fix is better?) Imho, once you have fixed in Formalin ( depending on the time in fixing fluid), you cannot successfully "refix" in another fixative. Also: "embedding": do you mean in OCT or Pwax? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________ From: [email protected] <[email protected]> Sent: 04 June 2018 18:00 To: [email protected] Subject: Histonet Digest, Vol 175, Issue 3 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0 or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Bouin postfixation (SANCHEZ QUINTEIRO PABLO) ---------------------------------------------------------------------- Message: 1 Date: Sun, 3 Jun 2018 18:00:43 +0000 From: SANCHEZ QUINTEIRO PABLO <[email protected]> To: "[email protected]" <[email protected]> Subject: [Histonet] Bouin postfixation Message-ID: <am0pr04mb39856cd03f81ca6cd26fa42291...@am0pr04mb3985.eurprd04.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" Dear histonetters, I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin. Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding? Thanks in advance Pablo Sanchez-Quinteiro Lugo. Spain ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list [email protected] https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 175, Issue 3 **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
