Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it!
-- Bharti Parihar, HT (ASCP)CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet