Davidson’s fixative is suggested to for use to fix in up to 24 hours. The small nodes should be at least halved and larger nodes cut no more than 2-3 mm thick. There are a few papers that state “small biopsies can be fixed rapidly”. I suggest < 6 hours for small biopsies. I would do, minimally, a quick verification study on multiple tissue samples before risking diagnostic samples. Hope that helps. William
William DeSalvo ________________________________ From: Azam, Muhammad via Histonet <histonet@lists.utsouthwestern.edu> Sent: Monday, July 30, 2018 10:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Davidson Hi; I am wondering what is the optimal time for Davidson's fixative (range). This is in regards to a sectioned lymph node. Thanks; Muhammad Azam, MD Staff Pathologist VHAMOU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Ccc9e5c75a99c490571f608d5f645a93f%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636685701408677759&sdata=KkIsiKg5Gb4Z95VrNryIIb%2B232gTbahSY%2Bt5kMYbrD0%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
