I enjoy this forum so much, I get to use brain cells I haven't used in years! When I saw your question about other counterstains for Iron Stain I kept thinking quick dip in Eosin (from you H&E) because I thought I remember having to do that in the 90's when we ran our of NFR. Histologist can be so resourceful with the right support. If I remember correctly light Eosin can also be used as a counterstain for GMS, some have used a very pale Hematoxylin but I personally don't care for it because it wasn't enough contrst for me. Tony, thanks for speaking up, it's nice to know I haven't forgot eveything with all the automation.
Cassie Davis Message: 6 Date: Wed, 8 Aug 2018 23:29:27 +0000 From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> To: "Campbell, Tasha M." <tmcam...@fmh.org> Cc: "firstname.lastname@example.org" <email@example.com> Subject: Re: [Histonet] Iron Stain Message-ID: <4125cb6319d24437bbd26ff12d106...@svdcmbx-mex024.nswhealth.net> Content-Type: text/plain; charset="us-ascii" Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet