When I was doing them biopsies ( pre-operative) to establish status, we used a std histochemical method on frozen sections of biopsy material ( visualising acetylcholinesterase activity). It used Osmium to enhance /darken positivity so, NOT recommended these days. I recall somebody leaving the lid off the Osmium ( stored at 4C)…..the fridge plastic lining was BLACK the next day!
So, any antibody that identifies nerve fibres and ganglion cells should be equally effective, using IHC/IF? Sure, interpretation is critical ( identifying presence/absence of ganglion cells and "significant" increase in lamina propria of nerve fibres as compensatory mechanism) There must be an internationally recognised/accepted protocol? Which technique does Great Ormond Street Hospital/St Thomas' Hospital use, these days? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
