Hi Jennifer, If it is skin extending the fixing and processing time should cure any processing issues. If after this it’s still not working a short pre fix in khales fixative and surface softening before sectioning with 0.02% sodium hydroxide may help.
Hope this helps! Stuart Beaver BSc(Hons) Head of Veterinary Histology/Cytology +447568543761 On 5 Sep 2018, at 22:46, P Sicurello <[email protected]<mailto:[email protected]>> wrote: Jennifer, I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue. What is it that is making them hard to cut? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet < [email protected]<mailto:[email protected]>> wrote: Hello Histonetters, Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. Thanks for any help, Jennifer Phinney QIHC Kansas State University Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list [email protected]<mailto:[email protected]> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
