Hi Lisa, We keep them in solution at 56-60 degrees overnight, and typically start differentiation between the 15-20 hour range.
Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -----Original Message----- From: Chapman, Lisa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, September 12, 2018 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luxol fast Blue Question (EXTERNAL EMAIL) Hello All, When performing a LFB for myelin and nissl, how long does everyone keep their slides in the LFB solution for and what temp do you use? Most procedures say "overnight" and Sheehan states 16-24 hours. Any advice would be greatly appreciated! Thank you in advance! Lisa Chapman HT (ASCP) ACL Laboratories Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
