I have always "fixed" in RT acetone for 10 mins Have compared 0-60 mins/4C to RT acetone Lower temps just limit the rate of reaction, imho. I note "nuclear streaming" when I use acetone at any temp/time.
Imho, acetone is not an effective fixative....it's a delipidiser. So, give it 10 mins at RT. If anyone thinks it's effective because it is a dehydrant….well, one re- hydrates the section to carry out IHC/ICC/IF. It works very well for only a few abs. When testing new abs out on FS/cell monolayers ( unfixed) I always compare Formalin, Acetone, Methanol and methanol/acetone 1:1 Imho Best wishes to a great site/membership. Carl _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
