Have you checked your dewaxing solutions? I have sometimes seen this when slides are not fully dewaxed.
yours, mills ᐧ On Wed, Nov 14, 2018 at 12:34 PM MONICA D. LOCKHART via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histo Fam!! > > Please help! > > My facility is experiencing frequent H & E staining problems. Everything > from cloudy cells on routine GI to "The Blank Look" on PNB's. > > We have made changes in some of the obvious areas like cassette whole size > and discontinued the use of sponges at grossing. However, with our > biopsies, we still see varying results in our H&E staining. > > Though this is not an exhaustive list of all the things we have > encountered or the number of changes we have made, hopefully this will give > you an idea of what we are seeing and maybe you have ran across this > problem and can share your wisdom. > > Any comments would be appreciated and most welcome. > > > Monica D. Lockhart, BBA, HT (ASCP) PBT > Supervisor Clinical Labs Histology > Loyola University Medical Center > 2160 S. First Ave, Bldg 110 Rm 2290 > Maywood, IL 60153 > (o) 708.327.2608 > (c) 708.692.8361 > monica.lockh...@luhs.org > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet