Hello, An update on my question:
On Fri, Nov 9, 2018 at 12:22 PM <histonet-requ...@lists.utsouthwestern.edu> wrote: > > Message: 1 > Date: Thu, 8 Nov 2018 15:45:35 -0600 > From: Tyrone Genade <tgen...@gmail.com> > To: histonet <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] counter stains for Sudan Black B > Message-ID: > < > caeyee3nxb7nwezrbl_uodtntjtqidhryxksdmgv4kvmv_pq...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello, > > I need a counterstain for use together with Sudan Black B. I am using this > method: https://www.ncbi.nlm.nih.gov/pubmed/27812872 which uses Nuclear > Fast Red. I don't have nuclear fast red... In an updated protocol by these > authors they use hematoxylin but in a previous trial I found it was too > dark and make visualizing the Sudan Black signal too difficult. I'm using > Sigma's Mayer's Hematoxylin solution. It is past its sell-by date and just > won't blue nicely any more.. > > I have available Methyl Green, Neutral Red and Safranin O. The former > washes out in aqueous/glycerol mounting medium (required for the protocol). > How well does Neutral Red and Safranin work with an aqueous mounting > medium? > There were no replies but Neutral Red worked. Does anyone know of a paper describing the cellular structures stained by Neutral red? https://en.wikipedia.org/wiki/Neutral_red states that is stains the Golgi apparatus and Nissl bodies and that living cells take up the stain in lysosomes. http://stainsfile.info/StainsFile/stain/nuclei/neutred.htm says it stains the nucleus. I see a darker stained red halo around the nucleus and the nucleus is stained reddish. It also seems to stain the aggresome, that is it stains small structures juxtaposed to the nucleus. The Sudan Black seems to overshadow the stain. Any speculation what is going on? Is the neutral red staining structures that would also contain lipofuscin? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Don't panic if I don't reply to your emails sent over the weekend. I don't usually have time to check my email over the weekend and will get back to you on Monday.* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet