Hi Curt, You can de-stain the hematoxylin counter stain and then do H&E and all should be fine. Subsequent IHC is possible but obviously you would need a different color chromogen to differentiate the new stain from the previous one.
There may be a problem getting the section stay on through a second IHC procedure but maybe not. I would stain a control section for the desired Ab using the retrieval method that was used initially and see how the control looks. If the pathologist thinks the that this control is adequate for interpretation, then restain the slides with the desired Ab and no additional retrieval. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet