John Garratt notes: >>Be aware that validation of IHC should be performed if you are changing your processing protocol by adding a dye to the reagents or to a pre-processed tissue. Be cautious!<<
I don't think safranin O would be a problem, but a good idea nonetheless! On Mon, Jan 14, 2019 at 6:40 PM John Garratt <john.garr...@ciqc.ca> wrote: > Be aware that validation of IHC should be performed if you are changing > your processing protocol by adding a dye to the reagents or to a > pre-processed tissue. Be cautious! > > > John > > > > On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to > > make sure they're recovered during embedding. > > > > I've had good results marking small specimens with the solution of > safranin > > O that's used in the microbiologists' Gram stain. Go to the micro lab and > > ask for a small amount of it and try it. > > > > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence > makes > > it very difficult to do any kind of fluorescent stain on the sections. > (It > > also doesn't work as well as safranin, which isn't fluorescent.) > > > > Another necessary procedure at the gross desk: fill out a log sheet that > > records the number of specimens you put into the cassette, and have that > > log sheet in front of you when you embed. (I've had a lot of histotechs > > flatly refuse to do this.) > > > > I like those little blue foam pads you put in the cassette and put the > > small specimens on. I usually cut them in two before putting them in the > > cassette. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet