There are many reasons why the nuclei may look dark in the Pap stain. 1. What type of hematoxylin are you using? 2. What type of Pap stain are you using, regressive or progressive?
This could be due to a variety of things like: type of preparation utilized, too long in hematoxylin, not enough rinsing of excess hematoxylin, not enough time in HCL (if used), too long in bluing, etc., personal preference of interpreting cytotech or pathologist, just to name a few. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewal...@rrmc.org, www.rrmc.org -----Original Message----- From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, March 19, 2019 3:18 PM To: Histo List <histonet@lists.utsouthwestern.edu> Subject: [Histonet] PAP stains [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. What causes dark nuclei in the PAP stain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cf0aa0f07b6854504f31408d6ac9fac74%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636886199125961814&sdata=Attb%2Fexy1igPSs6MNN3nWs8AwHgRBSjYKHOKpr7MXzM%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet