Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research
Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030
Office: 832-355-6524 | Fax: 832-355-6812
Email: [email protected]
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-----Original Message-----
From: Manfre, Philip via Histonet <[email protected]>
Sent: Tuesday, September 10, 2019 6:57 AM
To: Bob Richmond <[email protected]>; [email protected]
Subject: Re: [Histonet] Mallory-Azan stain
*** Important*** This email is not from Texas Heart Institute. Only click links
or open attachments you know are safe.
________________________________
The Bielschowsky Silver Stain is a good stain for Purkinje cells, so perhaps
fibers as well.
Phil.
Philip Manfre, BA, HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP81-406
770 Sumneytown Pike
West Point, PA 19486
215-652-9750
[email protected]
-----Original Message-----
From: Bob Richmond via Histonet <[email protected]>
Sent: Monday, September 9, 2019 1:24 PM
To: [email protected]
Subject: Re: [Histonet] Mallory-Azan stain
EXTERNAL EMAIL – Use caution with any links or file attachments.
Betsy Molinari HT, ASCP at the Texas Heart Institute in Houston asks:
>>I have a researcher that wants to stain Purkinje fibers and has
>>requested
a Mallory-Azan stain. I have no experience with this stain. I have looked
online for information but am reaching out to you for personal advice.<<
Well, I'm 80 years old and have never seen a Mallory-Heidenhain azan stain
- it was something you read about in old books when I was young. I have a fair
collection of old books, and found a method in Ann Preece's venerable _A Manual
for Histologic Technicians_, 3rd ed, 1972. I can scan it for you if nobody else
sends you one.
The stain requires aniline ("aniline oil"), which I don't think you're allowed
to handle any more - it's a significant hazmat.
Your researcher is probably using a very old reference, and needs to be brought
into the 21st century.
Bob Richmond
Samurai Pathologist
Maryville TN
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