You can see past issues of Morbidly and Mortality Weekly Report (MMWR) at: https://www.cdc.gov/mmwr/index2019.html
For past issues, click on "weekly report" on the left hand side and you can get to a list of past issues. However, for the publication about cryostats, look under "Supplements" a bit lower on the page and choose the 2012 supplement issue (Vol 61) . It is in the one on Laboratory Safety. It has a lot more than just cryostat safety. https://www.cdc.gov/mmwr/preview/ind2012_su.html Scroll to the very bottom of the page to see the January supplement The full reference is: MMWR January 6, 2012 / Vol. 61 / Supplement / Pg. 1 - 105 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Sheeder, Christopher via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, September 26, 2019 8:00 AM To: jasonhause...@gmail.com; P Sicurello Cc: Histonet Subject: Re: [Histonet] Flammable Sprays in Cryostats I would like the link as well, if possible. Christopher Sheeder, HT(ASCP)QIHC Histology Supervisor | Department of Laboratories Seattle Children’s Hospital 4800 Sand Point Way NE Seattle, WA 98105 -----Original Message----- From: jasonhause...@gmail.com <jasonhause...@gmail.com> Sent: Thursday, September 26, 2019 6:01 AM To: P Sicurello <pat...@gmail.com> Cc: Akemi <akemiat3...@gmail.com>; Knutson, Deanne <dknut...@primecare.org>; Histonet <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Flammable Sprays in Cryostats I woukd like the link to that. I looked around a bit on the web but couldnt locate usinf search.Jason HauserSenior HistotechnologistThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Thu, Sep 26, 2019 7:56 AMTo: jasonhause...@gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsThis was copied from the Centers for Disease Control's Morbidity and Mortality Weekly Report (January 2012)5.9.2. Work at the open benchFrozen sections— Frozen sectioning is performed on fresh tissue and is a high-risk procedure for infectious exposure. Freezing tissue does not kill organisms, and the use of the cryostat cutting blade creates potentially dangerous aerosols. Discuss the true clinical necessity for frozen sectioning with the surgical team.— Although some cryostat instruments have a downdraft into the instrument, aerosols are dispersed into the room where the cutting takes place. Do not use freezing propellant sprays, which speed the freezing process by a few seconds and cause aerosolization of not only the tissue being frozen but also the tissues from previously cut specimens that are at the base of the instrument. Such procedures generate aerosol and droplet contamination, posing an infectious risk to all personnel in the area (56,79,82). The Clinical and Laboratory Standards Institute and others have recommended discontinuation of freezing sprays because they are not recommended by the manufacturers of cryostat instrumentation (2,79). Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.On Thu, Sep 26, 2019 at 4:37 AM jasonhause...@gmail.com <jasonhause...@gmail.com> wrote: I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhause...@gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat. It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies. I would not recommend it, just as a universal precaution.Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet <histonet@lists.utsouthwestern.edu> wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > I just received a letter from Leica Biosystems where they are > prohibiting the usage of flammable freezing sprays in their cryostats. What > are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknut...@primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. 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