Hi Subash Imho your sections are not sufficiently adherent to the slide(s) It is not M/W "bubbling" that causes your problem If you use Superfrost Plus/lab-coated Silane or even double subbed slides ( thanks Ole!) AND dry your sections onto the slides efficiently, you will not get section-loss. NB: SF+ are expensive: if you cannot afford them....experiment with in-house Silane ( APES) or subbing, to coat your IHC slides. If you use the latter methods, make sure you slides are very clean. Unless the tissue is very fibrous/bone/cartilage/severely inflamed....difficult to keep sections adherent. In the latter case, Trajan 3 series can help much more than std coated slides. What tissues are causing you problems? For most soft tissues I use Superfrost Plus slides, dry the mounted sections under a fan O/N ( at least one hour) For my peace of mind I then put the slides into a 60C oven for 30-60 mins before dewaxing. Please post your opinion/results so far? Best wishes Carl
Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
