---------- Forwarded message --------- From: lucius lo <[email protected]> Date: Fri, 21 Feb 2020 at 15:46 Subject: Sectioning mice vertebrae and tail To: <[email protected]>
To whom may concern, I am a University student who currently pursuing a field in histology technician and is having a final year of the histology of bone marrow adipocytes in transgene mice. My question is regarding the sectioning of these fat-rich tissues which torn off easily. My way of sectioning is: 1. Freeze the blocks for an hour in wet ice immersed in distilled water. 2. Trim off the block in one micron while pressing down the lever. 3. Section the slide in five or six microns, then freeze the section again after trimmed off a hundred microns. 4. Obtain another section after placing the block in ice for 30 seconds. The torn off issue are highly corrected after I do step 4. However, *these tissues still appear with tiny holes that only appear after the H&E*. I would like to ask if anyone could share their experiences or solutions in facing tails and vertebrae. Best, Lucius _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
