Galina Deyneko (where? asks: >>Does anybody have experience how fix the tissues for successful glycogen ? detection in murine and humane cardiomyocytes. I am wondering maybe the trace of methanol in 10% formalin will dissolve glycogen?? - What would be better process for paraffin embedding or use OCT embedding without fixation? Of course I prefer FFPE blocks, since OCT blocks give bad morphology.<<
Ordinary neutral buffered formalin and paraffin embedding should be adequate. R.D. Lillie (3rd ed.) notes good results with Carnoy's fixative, alcoholic formalin, and acetic alcoholic formalin also. The traditional stain for glycogen is periodic acid Schiff (PAS). You verify the presence of glycogen by doing the stain with and without amylase ("diastase") predigestion. (A crude but adequate source of amylase is to just spit on the slide.) Bob Richmond Samurai Pathologist Maryville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet