Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections.
Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ken M via Histonet <[email protected]> Sent: Thursday, April 16, 2020 1:06 PM To: [email protected] Subject: [Histonet] Recommended thickness of Amyloid sections Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=zu56n7jP8NfTVcr03S3ER1yqzKYomhe2VIC5gz5CiHI&s=OTIHpP1NshAzBBAOzuP2Ljiz166ODsMhL_CDtbvQJgs&e= _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
