Hi Terri, The crystals form during cold winter months, usually during shipping or can form if the Hematoxylin 7211 is stored in a cold environment. They also form due to the Mordant being put into solution at it's saturation point. The crystals have no adverse effect on the stain itself. If your bottle has a few crystals, just filter it and it should remain crystal free. If there are a lot, you can gently warm it, using a "hot" plate and constant stirring until the crystals go back into solution, then filter it and is should be good to go. I only learned this when I "googled" about it myself. I hope this helps!!
Regards, Valerie Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 [email protected] www.parrishmed.com -----Original Message----- From: Terri Braud via Histonet [mailto:[email protected]] Sent: Thursday, July 09, 2020 12:39 PM To: [email protected] Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list [email protected] https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!vBWHyLqdMq6OOl66sorBVdmBIxsRePjH1moP6w3bOijwraARJ_4VQ8f_A8SaZWSs2RVXMg$ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
