Joanna, thanks! You are the first who said they had seen it before. I have a Leica tech coming in tomorrow to check out the syringes and lines. That was my thought too.
Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From: Bartczak, Joanna <joanna.bartc...@uhn.ca> Sent: Thursday, August 13, 2020 10:10 AM To: Paula Keene Pierce <pa...@excaliburpathology.com>; Morken, Timothy <timothy.mor...@ucsf.edu>; Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond I have seen this phenomenon years ago on the Bond III when I worked at a different laboratory. If I remember correctly, Leica had to replace the syringes and lines as air bubbles were being introduced when aspirating reagents. I would definitely contact Leica technical service and let them investigate. Good luck. Sincerely, Joanna Joanna Bartczak Charge Technologist - Immunopathology University Health Network - Toronto General Site 200 Elizabeth Street Toronto, Ontario M5G 2C4 Phone: (416) 340-4800 ext.6004 joanna.bartc...@uhn.ca<mailto:joanna.bartc...@uhn.ca> ________________________________ From: Paula Keene Pierce <pa...@excaliburpathology.com<mailto:pa...@excaliburpathology.com>> Sent: Wednesday, August 12, 2020 6:42 PM To: Morken, Timothy <timothy.mor...@ucsf.edu<mailto:timothy.mor...@ucsf.edu>>; Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond I have seen this when using Sequenza cover plates. My thoughts were that it is dissolved gases in the washes. As I noticed that when filling a container with my tap water and leaving it a while, bubbles form on the sides. Do you brush away bubbles in your water bath even when using distilled water too? Try not using freshly made, stirred, agitated, etc. solutions and adding a surfactant. Extreme solution: place solutions in a vacuum. Sent from Smallbiz Yahoo Mail for iPhone On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides and very noticeable on dual - stain slides on which the first stain is there but the second stain shows numerous bubbles that do prevent the second stain from areas of the tissue. We can see the bubble artifact on the slide away from tissue sections as well. Any clues to solve this will be appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CjcC7IQ!eYy1vmeWP5MP1WLTAt5O-QHHI3LnxW8O7vVLgSKZM005u_kiBtXjm1VAhKCf_Vw3cecE$<https://urldefense.com/v3/__http:/lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CjcC7IQ!eYy1vmeWP5MP1WLTAt5O-QHHI3LnxW8O7vVLgSKZM005u_kiBtXjm1VAhKCf_Vw3cecE$> This e-mail may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this e-mail may not be that of the organization. If you feel you have received an email from UHN of a commercial nature and would like to be removed from the sender's mailing list please do one of the following: (1) Follow any unsubscribe process the sender has included in their email (2) Where no unsubscribe process has been included, reply to the sender and type "unsubscribe" in the subject line. If you require additional information please go to our UHN Newsletters and Mailing Lists page. Please note that we are unable to automatically unsubscribe individuals from all UHN mailing lists. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
