Hi Scott, It may be best to call CAP to get an interpretation. However, I think that since you would be changing diluent for predictive markers, a full validation may be required.
Best, Dan Daniel Pesino, HTL(ASCP)QIHCCM Senior Histotechnologist Trinity Health Of New England daniel.pes...@trinityhealthofne.org W 860-714-4675 114 Woodland Street Hartford, CT 06105 TrinityHealthOfNE.org | Facebook | Twitter | Instagram -----Original Message----- From: Lindrud, Scott via Histonet <histonet@lists.utsouthwestern.edu> Sent: Thursday, March 25, 2021 8:30 AM To: Jeanine Ronkowski <jeanine.ronkow...@yahoo.com>; Paula Keene Pierce <pa...@excaliburpathology.com>; Maria Cruz <mcruz8...@gmail.com>; Morken, Timothy <timothy.mor...@ucsf.edu> Cc: Histonet <histonet@lists.utsouthwestern.edu> Subject: [External] Re: [Histonet] Antibody diluent Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. What is everyone's take on what needs to be done from a verification/validation standpoint if you switch diluents for your concentrated antibodies? The CAP summary of recommendations on the validation IHC antibodies doesn't specifically mention the changing of diluent as one of the variables. If I change the diluent on my ER and Her2 concentrates, do I need to do a complete re-validation or just a quick verification using a few positive and negative cases? I'm not really liking the idea of a complete re-validation! Thanks for any info! Scott Scott A. Lindrud, MLSCM(ASCP)CTCM | Histopathology Technical Specalist/Cytotechnologist P: 320-231-4520 F: 320-231-4503 scott.lind...@carrishealth.com Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 -----Original Message----- From: Jeanine Ronkowski <jeanine.ronkow...@yahoo.com> Sent: Tuesday, March 23, 2021 2:21 PM To: Paula Keene Pierce <pa...@excaliburpathology.com>; Maria Cruz <mcruz8...@gmail.com>; Morken, Timothy <timothy.mor...@ucsf.edu> Cc: Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Antibody diluent All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s really no such thing as a universal diluent like the ones offered by Leica and Agilent. We get ours from Biocare, they offer five different diluents with different pHs and chemical makeups and we use three of these. I checked with my Biocare rep and iit seems that the one that’s most similar to Leicas is called Monet Blue. I suggest you contact your Bocare rep and see if they can send you a sample of one or more them. Good luck,Jeanine Sent from Yahoo Mail for iPhone On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet <histonet@lists.utsouthwestern.edu> wrote: I just purchased antibody diluent from Electron Microscopy Sciences. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet <histonet@lists.utsouthwestern.edu> wrote: Maria, we went back to using Dako Diluent, which we had just switched away from to save money! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Maria Cruz via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, March 23, 2021 10:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody diluent Like other folks in the histo community, I’ve recently experienced the inability to buy diluent from Leica. Now wondering what others are doing to handle this problem. TIA! 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