Hi Colleen, I was involved in an Immunohistochemical study of the nerves in the human ureter that used Zamboni's fixative and cryostat sections. This study was before the invention of HIER.
If you are using Zamboni-fixed, paraffin embedded tissue then you will probably need to do HIER before many of the localisation antibody incubations since the fixative contains 2% formaldehyde. The same might be the case with some antibodies when applied to cryostat sections. Edyvane, K. A., Trussell, D. C., Jonavicius, J., Henwood, A., & Marshall, V. R. (1992). Presence and regional variation in peptide-containing nerves in the human ureter. Journal of the autonomic nervous system, 39(2), 127-137. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Colleen Forster via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, 26 March 2021 9:42 AM To: histonet-request <Histonet@lists.utsouthwestern.edu> Subject: [Histonet] IHC with zamboni's fixed tissues Hello Histonet, Can you give me feedback on trying to do IHC on samples fixed in Zamboni's fixative? I have an idea but need confirmation . Thank you in advance.... Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
