Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E? John Kiernan London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet <histonet@lists.utsouthwestern.edu> Sent: June 7, 2021 12:47 AM To: Gudrun Lang <gu.l...@gmx.at> Cc: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Prussian Blue Reaction
The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang <gu.l...@gmx.at> Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer <jmacdon...@mtsac.edu> Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0 -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Ursprüngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05IRM6WozVZiiR1g%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
