Martha, We reported our study of 15 brands of adhesive slides for "wash off" and found little difference among the different slides when well-fixed cell culture material was examined. On the other hand, poorly-fixed breast cancer tissues did appear to adhere more strongly to some slides than others (TOMO being among the best). Additional factors need to be considered, though, and I note that your baking procedure is different from what is recommended by many slide vendors. In general, baking at 60-65 deg C for 1 hour is said to be optimal, although we did not examine that parameter specifically.
Please see our poster at https://www.arrayscience.com/publications#Posters Best regards, Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 email: [email protected] www.arrayscience.com On Thu, Sep 23, 2021 at 1:02 PM Martha Ward-Pathology via Histonet < [email protected]> wrote: > It was brought to my attention that we had significant washing on 3 of 8 > bone marrow clot sections the other day; this is not the first time so we > would like to get to the bottom of this. We use positively charged slides > and all 8 cases were cut and run the same morning but allowed to air dry > and then bake at 60C for 20 minutes before being run on our Bond 3 > stainer. Has anyone out there experienced this type of problem and if so, > what were your solutions? The repeat of the 3 cases today showed similar > washing of tissue. > > This hasn't just started but has occurred periodically but the > pathologists have tried to live with it and usually we can finally get > enough tissue to stay on after 1-2 attempts. Suggestions include cutting > and drying the slides overnight and/or going to a gelatinated slide versus > a sialylated slide. We have been using this particular brand of positive > charged slide with good results for several years and rarely have issues > with other tissue types unless they are particularly bloody. > > Thoughts or suggestions are greatly appreciated. > > > Martha Ward MT(ASCP) QIHC > Atrium Health Wake Forest Baptist > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
