Imho....your waterbath is not hot enough The sections are not expanding Sure, if you are floating sections directly onto water bath....baaad Place your ribbon on a slide that has 20% alcohol on it Float that onto your waterbath The alcohol teases the sections nicely so....no creases. NB: Leave in the waterbath until you see no creases Works for me every time NB: I do many species of gut and have no problems if.....I do the above
Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
