Charles, Try and blot your sections dry before placing them into the oven. After picking up your section, place your slide onto a flat surface and take a paper towel or piece of filter paper, and gently press it down on top of the section to wick away all of the moisture. We use this technique frequently here on bone marrow cores.
Thanks, Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -----Original Message----- From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, August 30, 2022 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. I am working on trying to get IHC stains optimized on bone samples of rat tibias. The issue I am running into is that the periosteum is separating from the bone marrow and wrinkling up over top of the rest of the sample. If anyone has any techniques to prevent this wrinkling/detachment of tissue from the main section during automated ihc staining it would be greatly appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1srYnJjJBwOg5qJEJ_epFgbAXCjZWoirSFghf1tBQDTt4Kt86glQhWXDUFKHHe2BviKDOGHc_YOZrH3k3sKiGazjO1fRhU2GFJi18RGa2bb0f-VI2JMFwgoRCh3Mv4BFJnHqPjO4A8wLuoBUVteRZSMMDezrs0-eKxExtxe_Bn1OanJnxSFmzWIQXJl9XzUCwT1uCqSj9CFXMwW4swVLbFy3qpaq_74-LnLDO6yiQlBPyYP1upb0iwltU9iWQo-lfrXjFfKlYAHruMGUbJftFh1zBiA4sOKi90ubPDFxSvFr3-Aerb35g3yHJKIG5lZ2K0RuznvEbH-EjWmb7n3uhym4J61BoaaMBF1sH4MG7XG7yiiK5CIqm9SvxKGHyM5MeQXiTb_Ehnc74qW2NkSkEur2f06DKlv742nj6i9Ge70vNqu9tXdlIft0Q4nj4mBsO/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
