Paula, What does the artefact look like?
Another cause could be partial lifting of parts of the section, allowing reagents to become trapped under the section. You commonly see it in thyroids where the colloid traps antibody and/or detection reagents under the colloid giving a particulate brown dust-like staining (sometimes looking like cocci). You can also sometimes see it in fibrous tissue in sections. Suggestions to solve this: check your sticky adhesive slides, or allow the slides to dry longer at room temperature before placing on the Bond (allows the adhesive time to work). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
